Table 1. Diagnostic algorithms based on CBC analysis useful in HS screening.
| Parameters | Algorithm | Author (ref) |
|---|---|---|
| Ret (109/L)/IRF | >7.7 for HS | Mullier et al. (15) |
| >19 for Trait and mild HS | ||
| Micro R/Hypo-He | >2.5 for moderate HS if Hb is 8–12 g/dL | Mullier et al. (15) |
| >2 for severe HS, if Hb is <8 g/dL | ||
| Micro R | >3.5% for moderate and severe HS | Mullier et al (15) |
| Delta (MCV-MSCV) | >9.6 fl for HS, after excluding AIHA | Broséus et al. (19) |
| MSCV, MCV and MRV | HS highly probable if MSCV <70.2 or delta (MCV-MSCV) >10.4 and/or MRV <96.7 fl with positive CHT | Lazarova et al. (20) |
| MSCV, MCV and MRV | Delta (MCV-MSCV) >10 fl and delta (MRV-MSCV) <25 for HS differentiation from AIHA | Arora et al. (22) |
| MSCV, MCV and MRV | Delta (MCV-MSCV) >10 fl and delta (MRV-MSCV) <25 for HS | Nair et al. (28) |
| %Hyper | %Hyper >6.4% for HS | Farias et al. (24) |
| Ret (109/L) and MicroR | Ret ≥100×109/L and MicroR ≥2.6% for HS | Persijn et al. (26) |
CBC, complete blood count; HS, hereditary spherocytosis; Ret, reticulocytes count; IRF, immature reticulocytes fraction; MicroR, number of microcytic red blood cells; Hypo-He, RBCs with low hemoglobin concentration; MCV, mean corpuscular volume; MSCV, mean sphered corpuscular volume; MRV, mean reticulocyte volume; %Hyper, percentage of hyperdense cells.