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. 2018 Oct 1;9:1389. doi: 10.3389/fphys.2018.01389

FIGURE 2.

FIGURE 2

Double substitutions of N117C and N167C cause an LOF phenotype. (A) Enlarged superimposing view of N117 in TM3b and N167 in Cyto-helix depicted in a space-filling model from three crystal structures (PDB codes 2OAU, 2VV5, and 4HWA). Yellow, 2OAU; blue, 2VV5; gray, 4HWA. (B) Growth of the MJF465 (Δmscl, Δmscs, Δmsck) strain harboring empty vector or MscS. The panels show the 5-μl drop-plates at each tenfold dilution (10-2 to 10-4) of cells treated with mock shock (Mock) or osmotic downshock (Shock). (C,D) Percentage viability of cells exposed to osmotic downshock relative to mock shock (mean ± SEM, n = 5). ∗∗p < 0.01, ∗∗∗p < 0.001 vs. WT MscS.