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. 2018 Oct 1;9:1389. doi: 10.3389/fphys.2018.01389

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Copyright © 2018 Wang, Tang, Wen, Hong, Hong and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Double substitutions of N117C and N167C inhibit the gating of MscS. (A) Representative patch clamp recordings from the same patch before and after addition of βME to the bath. Lower traces: applied negative pressure. MscS expressed in E. coli MJF465 strain. (B) Left: single-channel openings recorded from WT MscS and βME treated N117C/N167C (dashed horizontal lines indicate closed states); right: count vs. amplitude histograms. (C) Conductance of WT MscS and βME treated N117C/N167C determined at 20 mV membrane potential (mean ± SEM, n = 6).