FIGURE 7.

Urease qualitative assay in the germination media (A) and germinating seeds of one genotype (B) and quantitative assay in contrasting genotypes (C). For A, the Arnon Hoagland media from Petriplates having 25, 50, 75, and 100% germinated seeds of the genotype Panvel 1 were qualititively assayed in triplicates (A1–A3) in urea broth for external urease, using soil as the positive control and only urea broth as the negative control. For B, all the 66 germinated seeds from two-thirds germinated plate were transferred either intact or after crushing, to test tubes containing Arnon Hoagland media and phenol red for qualitative assay of urease in triplicates (B1–B3). For C, quantitative urease assays were performed using the extracts of germinating seeds of fast germinating genotypes Aditya and Nidhi to contrast them with slow germinating genotypes, Panvel and Vikramarya. C1 shows the mean urease activities between fast and slow germinating genotypes, whereas C2 shows the activities in individual genotypes. The data are categorized into statistically distinct classes as denoted by alphabetic characters above the bars. Bars with different alphabets are significantly different at P < 0.05.