TABLE 3.

SUMMARY OF RESULTS FOR MICROARRAY ANALYSIS OF STRESS RESPONSES

Array*	Targets	Treatments	Induced†	Reduced‡	99% Conf.§
1.2Kchip	1238	20Gy + 4h	38	11	0.63-2.37
	1238	6 h hypoxia	135	19	0.51-2.47
	1238	24 h hypoxia	202	8	0.55-2.38
5K chip#	5408	2 Gy + 3 h	67	21	0.68-2.32
	5408	2Gy + 24h	161	26	0.69-1.89

Results are summarized for microarray hybridizations conducted in ML-1 cells. Many of the results for rows 1 and 3 have been verified by quantitative single-probe hybridization.

^*Two different microarrays (chips) used contained only limited overlap in the genes represented.

^†The number induced refers to cDNA clones showing significant induction (> 99% confidence).

^‡For cDNA clones showing a significant reduction, values are shown for those having at least a twofold reduction in expression compared to untreated cells; all these values exceeded 99% confidence.

^§Values represent range of relative expression of irradiated sample compared to untreated control (e.g., only targets showing an increase in the relative mRNA level of 2.37-fold or more were scored as induced in the first row).

^#Preliminary results with larger microarray are shown, but have not been verified by single-probe hybridization.