TABLE 3.
SUMMARY OF RESULTS FOR MICROARRAY ANALYSIS OF STRESS RESPONSES
| Array* | Targets | Treatments | Induced† | Reduced‡ | 99% Conf.§ |
|---|---|---|---|---|---|
| 1.2Kchip | 1238 | 20Gy + 4h | 38 | 11 | 0.63-2.37 |
| 1238 | 6 h hypoxia | 135 | 19 | 0.51-2.47 | |
| 1238 | 24 h hypoxia | 202 | 8 | 0.55-2.38 | |
| 5K chip# | 5408 | 2 Gy + 3 h | 67 | 21 | 0.68-2.32 |
| 5408 | 2Gy + 24h | 161 | 26 | 0.69-1.89 |
Results are summarized for microarray hybridizations conducted in ML-1 cells. Many of the results for rows 1 and 3 have been verified by quantitative single-probe hybridization.
Two different microarrays (chips) used contained only limited overlap in the genes represented.
The number induced refers to cDNA clones showing significant induction (> 99% confidence).
For cDNA clones showing a significant reduction, values are shown for those having at least a twofold reduction in expression compared to untreated cells; all these values exceeded 99% confidence.
Values represent range of relative expression of irradiated sample compared to untreated control (e.g., only targets showing an increase in the relative mRNA level of 2.37-fold or more were scored as induced in the first row).
Preliminary results with larger microarray are shown, but have not been verified by single-probe hybridization.