FIG. 2.

Northern mRNA steady-state analysis of LSAT and LSGC mRNAs in different cell lines. Cells were transfected with pLSGC or pLSAT plasmids, total cytoplasmic RNA was isolated, and equal amounts resolved by Northern analysis. S-antigen mRNA was hybridized to ³²P-labeled probes prepared from an S-antigen DNA fragment. Duplicate plates of cells were treated with 10 μM A23187 for 3 h prior to cell lysis.