FIG. 3.
Half-life of LSAT and LSGC mRNAs in HeLa cells treated with A23187. Cells were transfected with pLSGC or pLSAT plasmids, treated with 5 μM actinomycin D for the times indicated to block transcription. A23187 (10 μM) was added with actinomycin D to deplete plates of LSAT mRNA for 3 h. (A) Total cytoplasmic RNA was isolated, equal amounts resolved by Northern analysis and hybridized to 32P-labeled probes specific to the S-antigen or GAPDH coding region. Data were derived by averaging the densitometric plots of two independent trials. (B) Data are plotted relative to the untreated control mRNA (time 0), which was set to 1.0 for normalization.