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Effect of calcium ionophore A23187 on translation rates of reporter LSGC S-antigen mRNAs. Transfected HeLa and Cos cells, with and without 3-h treatment with 10 μM A23187, were labeled during treatment with 50 μCi of [35S]methionine per milliliter. Lysates were prepared and S-antigen immunoprecipitated from equal protein amounts and resolved by SDS-PAGE as described previously (1). S-antigen polypeptides consist of a 24-kDa species and a 27-kDa glycosylated form, as indicated.
