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. 2018 Sep 24;7(4-5-6):357–365.

FIG. 5.

FIG. 5

Kinetics and duration of LSAT mRNA stabilization by ionophore treatment of cells. HeLa cells were transfected with pLSGC or pLSAT plasmids. (A) Cells were treated with 10 μM A23187 for the times indicated. (B) Cells were treated with 10 μM A23187 for 30 min followed by a chase of new medium without A23187 for the times indicated. Total cytoplasmic RNA was purified, equal amounts resolved by Northern analysis and hybridized to probes for S-antigen or GAPDH mRNAs.