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HPLC fractionation of complex Ill-inducing activity. Partially purified complex Ill-inducing activity was loaded onto monoQ at 50 mM NaCl, washed with 100 mM NaCl, then eluted with a 100–500 mM NaCl gradient. Fractions were assayed for their ability to induce formation of complex III. Ø indicates the 25°C extract with no additions.
