Figure 7.

Peritoneal Macrophage from TRPC1^−/− Mice Exhibit Reduced M1 Inflammatory Responses during Experimental Peritonitis due to Klebsiella pneumoniae Infection

(A) Peritoneal macrophages from mock control (M) and KPn-infected WT and TRPC1^−/− mice were harvested. Western blot analysis using anti-pSTAT1, pp65, p65, STAT1, and GAPDH was performed on equal amount of the respective cell lysates. GAPDH, p65, and STAT1 were used as loading control. The bar graph (A′) depicts averages ± SEM of pixel intensity of the pSTAT1 and pp65 protein bands.

(B) Peritoneal macrophages from mock control (M) and KPn-infected WT and TRPC1^−/− mice were harvested, and the relative mRNA expression of M1 inflammatory mediators, NOS2, TNF-ᾳ, IL-6, IL-23, CXCL9, and CXCL10, and M2 anti-inflammatory mediators, CCL22 and ARG-1, was measured by qRT-PCR.

**p ≤ 0.01, ***p ≤ 0.001 (Student's t test).