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. 2018 Oct 6;16:56–61. doi: 10.1016/j.bbrep.2018.09.007

Fig. 2.

Fig. 2

QRTPCR validations of transcriptome changes in mSBC in response to IL-4. QRTPCR analysis of 15 genes, 14 tested for upregulation (Casp6, Cbr3, Cdh1, Cst7, Epcam, Grtp1, Lifr, Pmepa1, Pole2, Sertad4, Slc39a8, Tbxas1, Usp2, and Vdr) and 1 for downregulation (Sdc1). All the genes validated microarray results except Cbr3. QRTPCR data are expressed as 2–ΔΔCt. Pre, sample prior to culture; Ctrl and IL-4, samples cultured in parallel for 18 h with nothing and IL-4, respectively. Bars indicate mean ± S.D., ANOVA with Tukey's multiple comparison post test, ** p < 0.01, ***p < 0.001.