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. 2018 Sep 27;20:60–67. doi: 10.1016/j.redox.2018.09.013

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 2 — Reversibility of redox modulation of the enzyme activity of (A) HDAC8_wt and (B) HDAC8_C102S/C153S. The enzyme activity of the same HDAC8 sample after subsequent alternating addition of slight stoichiometric excesses of TCEP (red.) and H₂O₂ (ox.) is shown, respectively. The data represent means and standard deviations (HDAC8_wt: n = 15, HDAC8_C102S/C153S: n = 6). Statistical significance was determined by an unpaired t-test. ns: not significant (p > 0.05), *: 0.001 < p < 0.05 and ****: p < 0.0001.