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. 2018 Apr 11;94(5):651–657. doi: 10.1002/cyto.b.21630

Figure 1.

Figure 1

Gating strategy used by the software algorithm of BD HLA‐B27 assay on the BD FACSVia and BD FACSCalibur systems. On the BD FACSVia, initial gating of CD3+ T‐cells was placed in the CD3 PE (FL2) vs.SSC plot. On the BD FACSCalibur, initial gating of CD3+ T‐cells was placed in the FL2 (CD3) vs.FSC plot. LMF of HLA‐B27 FITC was obtained from the interval gate placed on all CD3+ T‐cells in the HLA‐B27 FITC (FL1) histogram. LMF was further compared to the predetermined decision marker using BD HLA‐B27 software algorithm to generate HLA‐B27 outcome. (A) Panel: HLA‐B27 positive sample (B) Panel: HLA‐B27 negative sample (C) Panel: Gray zone (Inconclusive) sample. In each panel, the same donor sample was analyzed using the same lot of BD HLA‐B27 kit on both the BD FACSCalibur and BD FACSVia systems. The decision marker of each reagent lot was set by the suffix on the reagent vial for HLA‐B27 FITC/CD3 PE.