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. Author manuscript; available in PMC: 2018 Oct 8.
Published in final edited form as: Mol Plant. 2017 Sep 8;10(10):1258–1273. doi: 10.1016/j.molp.2017.08.014

Figure 5. Transcriptional response to ambient warm temperature and to heat stress.

Figure 5

A Transcriptional patterns in Col-0 WT seedlings shifted from 22ºC to 27ºC. The clusters are in the same order as in Figure 1A. Transcriptional activation is also observed for genes in cluster 6 (highlighted with white box). Up-regulated genes are in yellow and down-regulated genes are in purple.

B Transcriptional patterns in Col-0 WT seedlings shifted from 12ºC to 37ºC. Transcriptional activation of the cluster 6 genes is stronger and persist longer when shifted to 37°C as compared to 27°C (highlighted with white box).

C Gene-by-gene HSFA1a ChIP signal of cluster 6 genes at 37°C. Further recruitment of HSF1 to the promoters and genes bodies of the cluster 6A genes resulted in elevated HSF1 occupancy at 37°C. Red dots indicate predicted HSEs from a known consensus motif.

D Dynamic profiles of HTA11 ChIP signal around the TTS of temperature-responsive genes Col-0 WT shifted from 12ºC to 37ºC. In plants shifted to 37°C, the H2A.Z eviction for cluster 6 genes (highlighted with white box) is stronger than shifting to 27°C and takes longer to be incorporated back into the nucleosomes (after 4 h). Green represents relative gain and blue represents relative loss of HTA11 ChIP signal between 17°C and 27°C.