Table 10.
Detailed results and statistical analyses related to the hyper-reactivity and escape behavior
| Reflexes and reactions to simple stimuli | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Data structure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vs Het | WT vs KO | Het vs KO | |
| Touch escape | 2wANOVA | Nonnormal | 1.26 ± 0.1 | 1.15 ± 0.11 | 2 ± 0.15 | 12.962 | 0.000 | 0.996 | 0.046 | 0.831 | 0.055 | 0.862 | 0.428 | 0.190 | 0.648 | 0.000 | 0.000 |
| Positional passivity (sum) | 2wANOVA | Nonnormal | 2.15 ± 0.25 | 1.84 ± 0.23 | 2.84 ± 0.2 | 11.737 | 0.000 | 0.992 | 106.722 | 0.000 | 1.000 | 1.993 | 0.147 | 0.393 | 0.034 | 0.011 | 0.000 |
| Positional passivity (score) | 2wANOVA | Nonnormal | 1.78 ± 0.24 | 2.21 ± 0.21 | 0.94 ± 0.2 | 14.029 | 0.000 | 0.998 | 44.935 | 0.000 | 1.000 | 3.871 | 0.027 | 0.675 | 0.034 | 0.004 | 0.000 |
| Catalepsy (4 trials) | 2wANOVA | Nonnormal | 2.98 ± 0.57 | 2.75 ± 0.54 | 0.56 ± 0.25 | 7.578 | 0.001 | 0.933 | 4.681 | 0.035 | 0.565 | 1.116 | 0.336 | 0.236 | 0.836 | 0.001 | 0.002 |
| Trunk curl | 2wANOVA | Nonnormal | 1 ± 0 | 0.89 ± 0.07 | 1 ± 0 | 2.547 | 0.088 | 0.487 | 2.537 | 0.117 | 0.346 | 2.547 | 0.088 | 0.487 | 0.057 | 1.000 | 0.056 |
| Negative geotaxis, latency to turn | 2wANOVA | Nonnormal | 6.73 ± 1.06 | 8.21 ± 1.9 | 3.22 ± 0.58 | 4.201 | 0.020 | 0.713 | 2.978 | 0.090 | 0.395 | 0.707 | 0.498 | 0.163 | 0.499 | 0.042 | 0.008 |
| Beam walking | |||||||||||||||||
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Data structure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vsHet | WT vsKO | Het vs KO | |
| Latency to cross (large, s) | 2wANOVA | Nonnormal | 8.34 ± 2.79 | 10.8 ± 4.52 | 3.96 ± 1.75 | 1.163 | 0.321 | 0.244 | 0.251 | 0.618 | 0.078 | 1.300 | 0.281 | 0.269 | − | − | − |
| Latency to cross (medium, s) | 2wANOVA | Nonnormal | 15.9 ± 4.8 | 7.17 ± 4.16 | 7.65 ± 6.13 | 0.811 | 0.450 | 0.181 | 3.349 | 0.073 | 0.435 | 0.121 | 0.887 | 0.068 | − | − | − |
| Latency to cross (small, s) | 2wANOVA | Nonnormal | 54.71 ± 5.89 | 46.15 ± 7.59 | 33.72 ± 7.84 | 2.204 | 0.121 | 0.430 | 0.235 | 0.630 | 0.076 | 0.437 | 0.648 | 0.117 | − | − | − |
| Escape behavior | |||||||||||||||||
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Datastructure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vs Het | WT vs KO | Het vs KO | |
| Buried food, number of attempts | 2wANOVA | Nonnormal | 0.63 ± 0.35 | 0.68 ± 0.32 | 0.42 ± 0.31 | 0.666 | 0.519 | 0.155 | 1.738 | 0.194 | 0.253 | 0.220 | 0.804 | 0.082 | |||
| Buried food, % of escapers | 2wANOVA | Nonnormal | 21.05 ± 9.6 | 26.31 ± 10.37 | 15.78 ± 8.59 | 0.760 | 0.473 | 0.172 | 3.639 | 0.062 | 0.464 | 0.159 | 0.853 | 0.073 | |||
| Four-object, number of attempts | 2wANOVA | Nonnormal | 0.41 ± 0.21 | 2.05 ± 0.73 | 3.88 ± 1.21 | 5.323 | 0.008 | 0.815 | 5.320 | 0.025 | 0.618 | 3.316 | 0.045 | 0.601 | 0.187 | 0.002 | 0.050 |
| Four-object, % of escapers | 2wANOVA | Nonnormal | 23.52 ± 10.6 | 36.84 ± 11.36 | 50 ± 12.12 | 1.502 | 0.233 | 0.305 | 4.351 | 0.042 | 0.534 | 2.575 | 0.087 | 0.490 | |||
| Marble burying, number of attempts | 2wANOVA | Nonnormal | 4.32 ± 1.28 | 10.63 ± 1.98 | 16.05 ± 2.38 | 8.063 | 0.001 | 0.946 | 6.649 | 0.013 | 0.715 | 1.239 | 0.299 | 0.257 | 0.034 | 0.000 | 0.055 |
| Marble burying, % of escapers | 2wANOVA | Nonnormal | 47.36 ± 11.76 | 94.73 ± 5.26 | 100 ± 0 | 12.009 | 0.000 | 0.993 | 7.713 | 0.008 | 0.777 | 4.474 | 0.017 | 0.740 | 0.000 | 0.000 | 0.598 |
WT, wild-type mice; Het, heterozygous mice; KO, homozygous knock-out mice. Group values are reported as mean ± SEM. Red font indicates significant results (p < 0.05). Individual results and statistical analyses for cohorts 1 and 2 are available in Extended Data Table 10-1. 2wANOVA: 2-way ANOVA.