Table 13.
Detailed results and statistical analyses related to anxiety-like behaviors
| Open field thigmotaxis | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Data structure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vs Het | WT vs KO | Het vs KO | |
| Border distance (cm) | 2wANOVA | Normal | 10507.07 ± 558.17 | 8848.23 ± 548.19 | 7942.17 ± 394.99 | 6.537 | 0.003 | 0.892 | 10.443 | 0.002 | 0.887 | 0.080 | 0.923 | 0.062 | 0.022 | 0.001 | 0.235 |
| Border distance (cm) | Test | Data structure | F | p value | Power | WT vs Het | WT vs KO | Het vsKO | |||||||||
| - Time | rMeasures | Sph.viol | 52.599 | 0.000 | 1.000 | − | − | − | |||||||||
| - Time × genotype | rMeasures | Sph.viol | 2.496 | 0.007 | 0.877 | − | − | − | |||||||||
| - Genotype | rMeasures | Sph.viol | 6.537 | 0.003 | 0.892 | 0.043 | 0.001 | 0.373 | |||||||||
| - Cohort | rMeasures | Sph.viol | 10.443 | 0.002 | 0.887 | − | − | − | |||||||||
| - Time × gen. × coh. | rMeasures | Sph.viol | 0.923 | 0.492 | 0.399 | − | − | − | |||||||||
| - Genotype × cohort | rMeasures | Sph.viol | 0.080 | 0.923 | 0.062 | − | − | − | |||||||||
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Data structure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vs Het | WT vs KO | Het vs KO | |
| Center distance (cm) | 2wANOVA | Nonnormal | 3276.58 ± 335.66 | 2390.56 ± 291.66 | 2139.7 ± 246.94 | 3.932 | 0.026 | 0.682 | 9.890 | 0.003 | 0.870 | 0.049 | 0.952 | 0.057 | 0.036 | 0.011 | 0.622 |
| Center distance (cm) | Test | Data structure | F | p value | Power | WT vs Het | WT vs KO | Het vs KO | |||||||||
| - Time | rMeasures | Sph.viol | 1.158 | 0.330 | 0.343 | − | − | − | |||||||||
| - Time × genotype | rMeasures | Sph.viol | 1.327 | 0.237 | 0.571 | − | − | − | |||||||||
| - Genotype | rMeasures | Sph.viol | 3.932 | 0.026 | 0.682 | 0.070 | 0.015 | 0.798 | |||||||||
| - Cohort | rMeasures | Sph.viol | 9.890 | 0.003 | 0.870 | − | − | − | |||||||||
| - Time × gen. × coh. | rMeasures | Sph.viol | 0.695 | 0.683 | 0.302 | − | − | − | |||||||||
| - Genotype × cohort | rMeasures | Sph.viol | 0.049 | 0.952 | 0.057 | − | − | − | |||||||||
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Data structure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vsHet | WT vs KO | Het vs KO | |
| Border/total distance | 2wANOVA | Normal | 76.87 ± 1.51 | 79.57 ± 1.69 | 79.69 ± 1.29 | 0.950 | 0.393 | 0.206 | 5.570 | 0.022 | 0.639 | 0.049 | 0.952 | 0.057 | − | − | − |
| Border/total distance | Test | Data structure | F | p value | Power | WT vs Het | WT vsKO | Het vs KO | |||||||||
| - Time | rMeasures | Sph.viol | 5.035 | 0.001 | 0.957 | − | − | − | |||||||||
| - Time × genotype | rMeasures | Sph.viol | 1.182 | 0.312 | 0.531 | − | − | − | |||||||||
| - Genotype | rMeasures | Sph.viol | 1.017 | 0.369 | 0.218 | − | − | − | |||||||||
| - Cohort | rMeasures | Sph.viol | 5.820 | 0.019 | 0.658 | − | − | − | |||||||||
| - Time × gen. × coh. | rMeasures | Sph.viol | 0.679 | 0.705 | 0.305 | − | − | − | |||||||||
| - Genotype × cohort | rMeasures | Sph.viol | 0.094 | 0.911 | 0.064 | − | − | − | |||||||||
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Data structure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vs Het | WT vs KO | Het vsKO | |
| Center/total distance | 2wANOVA | Normal | 22.88 ± 1.51 | 20.15 ± 1.68 | 20.16 ± 1.27 | 0.939 | 0.398 | 0.204 | 4.471 | 0.039 | 0.546 | 0.048 | 0.953 | 0.057 | − | − | − |
| Center/total distance | Test | Data structure | F | p value | Power | WT vs Het | WT vs KO | Het vsKO | |||||||||
| - Time | rMeasures | Sph.viol | 5.177 | 0.001 | 0.962 | − | − | − | |||||||||
| - Time × genotype | rMeasures | Sph.viol | 1.177 | 0.315 | 0.527 | − | − | − | |||||||||
| - Genotype | rMeasures | Sph.viol | 1.001 | 0.375 | 0.215 | − | − | − | |||||||||
| - Cohort | rMeasures | Sph.viol | 4.757 | 0.034 | 0.571 | − | − | − | |||||||||
| - Time × gen. × coh. | rMeasures | Sph.viol | 0.652 | 0.728 | 0.292 | − | − | − | |||||||||
| - Genotype × cohort | rMeasures | Sph.viol | 0.088 | 0.916 | 0.063 | − | − | − | |||||||||
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Data structure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vs Het | WT vs KO | Het vs KO | |
| Border/Center distance | 2wANOVA | Nonnormal | 3.78 ± 0.38 | 4.87 ± 0.74 | 4.34 ± 0.36 | 0.990 | 0.379 | 0.213 | 3.522 | 0.066 | 0.453 | 0.216 | 0.807 | 0.082 | − | − | − |
| Border/Center distance | Test | Data structure | F | pvalue | Power | WT vs Het | WT vs KO | Het vsKO | |||||||||
| - Time | rMeasures | Sph.viol | 5.177 | 0.001 | 0.240 | − | − | − | |||||||||
| - Time × genotype | rMeasures | Sph.viol | 1.177 | 0.315 | 0.456 | − | − | − | |||||||||
| - Genotype | rMeasures | Sph.viol | 1.001 | 0.375 | 0.309 | − | − | − | |||||||||
| - Cohort | rMeasures | Sph.viol | 4.757 | 0.034 | 0.469 | − | − | − | |||||||||
| - Time × gen. × coh. | rMeasures | Sph.viol | 0.652 | 0.728 | 0.196 | − | − | − | |||||||||
| - Genotype × cohort | rMeasures | Sph.viol | 0.088 | 0.916 | 0.230 | − | − | − | |||||||||
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Data structure | WT | Het | KO | F | p value | Power | F | pvalue | Power | F | pvalue | Power | WT vs Het | WT vsKO | Het vs KO | |
| Border time (s) | 2wANOVA | Normal | 2969.88 ± 70.88 | 2993.1 ± 77.46 | 3067.4 ± 62.2 | 0.481 | 0.621 | 0.124 | 1.088 | 0.302 | 0.176 | 0.582 | 0.563 | 0.141 | − | − | − |
| Border time (s) | Test | Data structure | F | p value | Power | WT vs Het | WT vs KO | Het vsKO | |||||||||
| - Time | rMeasures | Sph.viol | 2.960 | 0.023 | 0.773 | − | − | − | |||||||||
| - Time × genotype | rMeasures | Sph.viol | 0.836 | 0.568 | 0.374 | − | − | − | |||||||||
| - Genotype | rMeasures | Sph.viol | 0.481 | 0.621 | 0.124 | − | − | − | |||||||||
| - Cohort | rMeasures | Sph.viol | 1.088 | 0.302 | 0.176 | − | − | − | |||||||||
| - Time × gen. × coh. | rMeasures | Sph.viol | 0.792 | 0.606 | 0.354 | − | − | − | |||||||||
| - Genotype × cohort | rMeasures | Sph.viol | 0.582 | 0.563 | 0.141 | − | − | − | |||||||||
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Data structure | WT | Het | KO | F | p value | Power | F | pvalue | Power | F | p value | Power | WT vs Het | WT vs KO | Het vsKO | |
| Center time (s) | 2wANOVA | Normal | 612.08 ± 71.17 | 587.82 ± 77.5 | 517.59 ± 62.2 | 0.451 | 0.640 | 0.119 | 0.871 | 0.355 | 0.150 | 0.589 | 0.559 | 0.143 | − | − | − |
| Center time (s) | Test | Data structure | F | p value | Power | WT vs Het | WT vs KO | Het vsKO | |||||||||
| - Time | rMeasures | Sph.viol | 3.200 | 0.016 | 0.807 | − | − | − | |||||||||
| - Time × genotype | rMeasures | Sph.viol | 0.836 | 0.568 | 0.363 | − | − | − | |||||||||
| - Genotype | rMeasures | Sph.viol | 0.481 | 0.621 | 0.119 | − | − | − | |||||||||
| - Cohort | rMeasures | Sph.viol | 1.088 | 0.302 | 0.150 | − | − | − | |||||||||
| - Time × gen. × coh. | rMeasures | Sph.viol | 0.792 | 0.606 | 0.090 | − | − | − | |||||||||
| - Genotype × cohort | rMeasures | Sph.viol | 0.582 | 0.563 | 0.143 | − | − | − | |||||||||
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Data structure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vs Het | WT vs KO | Het vsKO | |
| Border/center time | 2wANOVA | Nonnormal | 6.95 ± 1.32 | 8.3 ± 1.88 | 9.76 ± 2.27 | 0.476 | 0.624 | 0.124 | 0.533 | 0.469 | 0.111 | 0.107 | 0.898 | 0.066 | − | − | − |
| Border/center time | Test | Data structure | F | p value | Power | WT vs Het | WT vs KO | Het vsKO | |||||||||
| - Time | rMeasures | Sph.viol | 0.290 | 0.822 | 0.103 | − | − | − | |||||||||
| - Time × genotype | rMeasures | Sph.viol | 1.575 | 0.163 | 0.575 | − | − | − | |||||||||
| - Genotype | rMeasures | Sph.viol | 0.429 | 0.653 | 0.116 | − | − | − | |||||||||
| - Cohort | rMeasures | Sph.viol | 1.546 | 0.220 | 0.230 | − | − | − | |||||||||
| - Time × gen. × coh. | rMeasures | Sph.viol | 0.575 | 0.740 | 0.219 | − | − | − | |||||||||
| - Genotype × cohort | rMeasures | Sph.viol | 1.594 | 0.213 | 0.322 | − | − | − | |||||||||
| Vertical activity in open field | |||||||||||||||||
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Data structure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vs Het | WT vs KO | Het vsKO | |
| Free rears duration (s) | 2wANOVA | Nonnormal | 4.66 ± 1.25 | 7.93 ± 1.62 | 6.18 ± 1.48 | 1.159 | 0.322 | 0.243 | 0.036 | 0.850 | 0.054 | 1.480 | 0.237 | 0.301 | − | − | − |
| Free rears number | 2wANOVA | Nonnormal | 8.42 ± 2.13 | 10.63 ± 1.66 | 7.57 ± 1.21 | 0.837 | 0.439 | 0.186 | 1.988 | 0.165 | 0.283 | 1.369 | 0.264 | 0.281 | − | − | − |
| Wall rears duration (s) | 2wANOVA | Nonnormal | 14.68 ± 1.77 | 16.13 ± 2.02 | 9.01 ± 0.84 | 5.023 | 0.010 | 0.793 | 1.924 | 0.171 | 0.275 | 0.397 | 0.675 | 0.111 | 0.805 | 0.045 | 0.009 |
| Wall rears number | 2wANOVA | Nonnormal | 27.26 ± 2.68 | 27.52 ± 2.86 | 19.36 ± 1.88 | 3.576 | 0.035 | 0.638 | 19.306 | 0.000 | 0.991 | 0.414 | 0.663 | 0.113 | 0.996 | 0.036 | 0.030 |
| All rears duration (s) | 2wANOVA | Nonnormal | 19.34 ± 2.3 | 24.07 ± 3.01 | 15.2 ± 1.99 | 3.140 | 0.052 | 0.578 | 0.646 | 0.425 | 0.124 | 1.240 | 0.298 | 0.258 | 0.374 | 0.468 | 0.038 |
| All rears number | 2wANOVA | Nonnormal | 35.68 ± 3.83 | 38.15 ± 3.94 | 26.94 ± 2.08 | 3.240 | 0.047 | 0.592 | 16.104 | 0.000 | 0.976 | 1.267 | 0.290 | 0.263 | 0.829 | 0.107 | 0.028 |
| Zero-maze | |||||||||||||||||
| Genotype | Cohort | Genotype ×cohort | Pairwise comparisons | ||||||||||||||
| Test | Datastructure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vs Het | WT vs KO | Het vs KO | |
| Closed arc time, d1 | 2wANOVA | Normal | 428.66 ± 18.61 | 441.68 ± 13.82 | 456.86 ± 18.31 | 0.400 | 0.672 | 0.111 | 13.684 | 0.001 | 0.952 | 0.311 | 0.734 | 0.097 | − | − | − |
| Closed arc time, d2 | 2wANOVA | Nonnormal | 448.33 ± 20.43 | 492.29 ± 16.58 | 487.59 ± 29.63 | 3.652 | 0.033 | 0.646 | 36.459 | 0.000 | 1.000 | 0.114 | 0.893 | 0.066 | 0.138 | 0.009 | 0.489 |
| Closed arc time, m | 2wANOVA | Normal | 438.5 ± 18.27 | 466.99 ± 13.34 | 472.23 ± 20.15 | 1.917 | 0.158 | 0.379 | 28.873 | 0.000 | 1.000 | 0.253 | 0.778 | 0.088 | − | − | − |
| Open arc time, d1 | 2wANOVA | Normal | 166.03 ± 18.25 | 153.09 ± 13.6 | 134.72 ± 17.99 | 0.562 | 0.574 | 0.138 | 10.417 | 0.002 | 0.886 | 0.420 | 0.659 | 0.114 | − | − | − |
| Open arc time, d2 | 2wANOVA | Nonnormal | 143.08 ± 21.29 | 102.14 ± 16.74 | 78.39 ± 17.83 | 3.063 | 0.056 | 0.566 | 37.315 | 0.000 | 1.000 | 0.151 | 0.860 | 0.072 | 0.194 | 0.015 | 0.502 |
| Open arc time, m | 2wANOVA | Normal | 154.55 ± 18.53 | 127.61 ± 13.25 | 106.56 ± 16.4 | 1.863 | 0.166 | 0.369 | 26.343 | 0.000 | 0.999 | 0.340 | 0.713 | 0.101 | − | − | − |
| Close/open time, d1 | 2wANOVA | Nonnormal | 4.08 ± 0.85 | 3.74 ± 0.98 | 5.76 ± 1.6 | 0.820 | 0.447 | 0.182 | 9.172 | 0.004 | 0.843 | 0.438 | 0.648 | 0.117 | − | − | − |
| Close/open time, d2 | 2wANOVA | Nonnormal | 6.95 ± 1.95 | 25.36 ± 18.5 | 28.98 ± 11.02 | 0.969 | 0.387 | 0.209 | 5.901 | 0.019 | 0.663 | 0.746 | 0.479 | 0.169 | − | − | − |
| Close/open time, m | 2wANOVA | Nonnormal | 4.61 ± 1 | 5.55 ± 1.95 | 8.57 ± 2.42 | 1.207 | 0.308 | 0.251 | 11.378 | 0.001 | 0.911 | 0.538 | 0.587 | 0.134 | − | − | − |
| Open arc entries, d1 | 2wANOVA | Nonnormal | 48.47 ± 4.88 | 50.52 ± 4.46 | 59.36 ± 7.73 | 1.870 | 0.165 | 0.371 | 14.588 | 0.000 | 0.963 | 0.603 | 0.551 | 0.145 | − | − | − |
| Open arc entries, d2 | 2wANOVA | Normal | 42.42 ± 5.18 | 36.52 ± 4.83 | 29.31 ± 5.07 | 1.238 | 0.299 | 0.257 | 19.130 | 0.000 | 0.990 | 0.137 | 0.872 | 0.070 | − | − | − |
| Open arc entries, m | 2wANOVA | Normal | 45.44 ± 4.16 | 43.52 ± 4.04 | 44.34 ± 5.81 | 0.150 | 0.861 | 0.072 | 24.720 | 0.000 | 0.998 | 0.447 | 0.642 | 0.119 | − | − | − |
| Open entering arc latency | 2wANOVA | Nonnormal | 39.29 ± 13.16 | 47.08 ± 31.35 | 22.99 ± 10.38 | 0.310 | 0.735 | 0.097 | 0.684 | 0.412 | 0.128 | 1.282 | 0.287 | 0.265 | − | − | − |
| Open arc crossing latency | 2wANOVA | Nonnormal | 149.34 ± 34.87 | 139.69 ± 35.2 | 95.2 ± 32.99 | 0.796 | 0.457 | 0.178 | 1.515 | 0.224 | 0.226 | 0.395 | 0.676 | 0.110 | − | − | − |
| Close arc dipping number, d1 | 2wANOVA | Nonnormal | 51.73 ± 6.81 | 55.42 ± 6.48 | 51.47 ± 7.31 | 0.303 | 0.740 | 0.096 | 54.807 | 0.000 | 1.000 | 0.035 | 0.965 | 0.055 | − | − | − |
| Close arc dipping number, d2 | 2wANOVA | Nonnormal | 31.94 ± 3.01 | 28.94 ± 3.69 | 22.21 ± 3.87 | 2.182 | 0.124 | 0.425 | 54.920 | 0.000 | 1.000 | 0.593 | 0.556 | 0.143 | − | − | − |
| Close arc dipping number, m | 2wANOVA | Nonnormal | 41.84 ± 4.31 | 42.5 ± 4.48 | 36.84 ± 5.05 | 0.239 | 0.788 | 0.085 | 94.671 | 0.000 | 1.000 | 0.179 | 0.836 | 0.076 | − | − | − |
| Close arc dipping time, d1 | 2wANOVA | Nonnormal | 131.15 ± 14.57 | 145.82 ± 14.24 | 117.88 ± 13.74 | 0.984 | 0.381 | 0.211 | 57.892 | 0.000 | 1.000 | 0.648 | 0.527 | 0.153 | − | − | − |
| Close arc dipping time, d2 | 2wANOVA | Nonnormal | 94.51 ± 10 | 100.08 ± 13.98 | 64.33 ± 12.46 | 2.111 | 0.132 | 0.413 | 26.004 | 0.000 | 0.999 | 0.342 | 0.712 | 0.102 | − | − | − |
| Close arc dipping time, m | 2wANOVA | Normal | 112.83 ± 11.57 | 124.49 ± 12.37 | 91.1 ± 10.18 | 2.386 | 0.103 | 0.459 | 72.828 | 0.000 | 1.000 | 0.811 | 0.450 | 0.181 | − | − | − |
| Open arc dipping number, d1 | 2wANOVA | Nonnormal | 27.15 ± 3.63 | 20.26 ± 2.46 | 19.15 ± 3.92 | 1.441 | 0.247 | 0.294 | 8.140 | 0.006 | 0.799 | 0.567 | 0.571 | 0.139 | − | − | − |
| Open arc dipping number, d2 | 2wANOVA | Nonnormal | 16.94 ± 3.04 | 12.22 ± 2.34 | 8.84 ± 2.54 | 1.938 | 0.155 | 0.383 | 17.131 | 0.000 | 0.982 | 0.332 | 0.719 | 0.100 | − | − | − |
| Open arc dipping number, m | 2wANOVA | Nonnormal | 22.05 ± 3.15 | 16.97 ± 2.15 | 14 ± 2.9 | 2.060 | 0.138 | 0.404 | 15.216 | 0.000 | 0.969 | 0.502 | 0.609 | 0.128 | − | − | − |
| Zero-maze | |||||||||||||||||
| Genotype | Cohort | Genotype ×cohort | Pairwise comparisons | ||||||||||||||
| Test | Datastructure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vs Het | WT vs KO | Het vs KO | |
| Open arc dipping time, d1 | 2wANOVA | Nonnormal | 62.85 ± 10.83 | 42.28 ± 5.15 | 25.57 ± 5.27 | 5.700 | 0.006 | 0.843 | 13.928 | 0.000 | 0.955 | 1.730 | 0.188 | 0.346 | 0.091 | 0.001 | 0.245 |
| Open arc dipping time, d2 | 2wANOVA | Nonnormal | 51.3 ± 10.52 | 35.3 ± 8.44 | 17.87 ± 5.34 | 3.798 | 0.029 | 0.665 | 20.104 | 0.000 | 0.993 | 0.427 | 0.655 | 0.115 | 0.278 | 0.008 | 0.269 |
| Open arc dipping time, m | 2wANOVA | Nonnormal | 57.07 ± 9.63 | 39.17 ± 5.86 | 21.72 ± 4.71 | 6.448 | 0.003 | 0.886 | 23.422 | 0.000 | 0.997 | 1.299 | 0.282 | 0.268 | 0.088 | 0.001 | 0.157 |
| Time open vs close, d1 | Test | Data structure | All F | All p value | Power | WT F | WT p value | Power | Het F | Het p value | Power | KO F | KO p value | Power | |||
| - Zone | rMeasures | Sph.ass | 277.319 | 0.000 | 1.000 | 73.861 | 0.000 | 1.000 | 125.301 | 0.000 | 1.000 | 83.255 | 0.000 | 1.000 | |||
| - Cohort | rMeasures | Sph.ass | 8.156 | 0.006 | 0.935 | 5.563 | 0.031 | 0.604 | 8.257 | 0.011 | 0.773 | 3.298 | 0.087 | 0.403 | |||
| - Zone × cohort | rMeasures | Sph.ass | 12.518 | 0.001 | 0.801 | 7.248 | 0.015 | 0.718 | 3.063 | 0.098 | 0.379 | 2.143 | 0.161 | 0.282 | |||
| Time open vs close, d2 | Test | Data structure | All F | All p value | Power | WT F | WT p value | Power | Het F | Het p value | Power | KO F | KO p value | Power | |||
| - Zone | rMeasures | Sph.ass | 440.281 | 0.000 | 1.000 | 94.767 | 0.000 | 1.000 | 278.317 | 0.000 | 1.000 | 119.843 | 0.000 | 1.000 | |||
| - Cohort | rMeasures | Sph.ass | 0.578 | 0.450 | 0.218 | 1.269 | 0.276 | 0.186 | 2.497 | 0.132 | 0.320 | 0.921 | 0.351 | 0.148 | |||
| - Zone × cohort | rMeasures | Sph.ass | 25.848 | 0.000 | 1.000 | 12.054 | 0.003 | 0.905 | 18.624 | 0.000 | 0.982 | 3.749 | 0.070 | 0.447 | |||
| Time open vs close, m | Test | Data structure | All F | All p value | Power | WT F | WT pvalue | Power | Het F | Het pvalue | Power | KO F | KO pvalue | Power | |||
| - Zone | rMeasures | Sph.ass | 440.281 | 0.000 | 1.000 | 103.409 | 0.000 | 1.000 | 274.392 | 0.000 | 1.000 | 131.582 | 0.000 | 1.000 | |||
| - Cohort | rMeasures | Sph.ass | 0.578 | 0.450 | 0.116 | 0.006 | 0.941 | 0.051 | 0.422 | 0.524 | 0.094 | 0.484 | 0.496 | 0.101 | |||
| - Zone × cohort | rMeasures | Sph.ass | 25.848 | 0.000 | 0.999 | 11.720 | 0.003 | 0.897 | 12.720 | 0.002 | 0.919 | 3.786 | 0.068 | 0.451 | |||
WT, wild-type mice; Het, heterozygous mice; KO, homozygous knock-out mice. Group values are reported as mean ± SEM. Bold font indicates significant results (p < 0.05). Individual results and statistical analyses for cohorts 1 and 2 are available in Extended Data Table 13-1. 2wANOVA: 2-way ANOVA, rMeasures: repeated measures, Norm: normal, No-norm: non-normal, Sph.ass: sphericity assumed, Sph.viol: sphericity violated, gen: genotype, coh: cohort, d1: day 1, d2: day 2, m: day 1 - day 2 mean. 2wANOVA: 2-way ANOVA, rMeasures: repeated measures, Sph.ass: sphericity assumed, Sph.viol: sphericity violated, gen: genotype, coh: cohort, d1: day 1, d2: day 2, m: day 1 - day 2 mean.