Figure 5.

Urinary excretion of sulforaphane and metabolites (μmoles) in 24 h following consumption of Myb28^B/B (84 μmoles glucoraphanin per 300 g soup), Myb28^B/V (280 μmoles glucoraphanin per 300 g soup), and Myb28^V/V (452 μmoles glucoraphanin per 300 g soup) broccoli soups. Cumulative excretion (A) and total urinary excretion (B) of sulforaphane and metabolites in urine samples analyzed by LC–MS/MS. Samples were analyzed for sulforaphane metabolites including erucin‐NAC, sulforaphane‐cysteine, sulforaphane–cysteine‐glycine, and sulforaphane‐NAC. Data (n = 10) are represented as mean ± SD. Total urinary excretion data underwent log root transformation followed by analysis by one‐way analysis of variance with Tukey's honest significance test (*p < 0.05 and ***p ˂ 0.001 vs My28B/B broccoli soup).