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. 2018 Sep 18;20(9):3343–3362. doi: 10.1111/1462-2920.14339

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© 2018 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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H3K4me3 and H3K4me2 levels at gibberellic acid (GA) cluster genes in Δset1, Δkdm5 and OE::KDM5 mutants. Indicated strains were grown in liquid culture (ICI + 6 mM Gln) for 3 days prior to ChIP‐qPCR using the (A) H3K4me3 or (B) H3K4me2 antibody. Therefore, the enriched samples (precipitated by antibody) were normalized to the respective input samples (initially applied chromatin). The WT was arbitrarily set to 1, and the data are mean values ± SD (n = 4). For statistical analysis, the mutants were compared with the WT using the student's t‐test: *, p < 0.05; **, p < 0.01.