Figure 3.

Effects of l‐AHG on tyrosinase activity in vitro. Effects of l‐AHG on in vitro tyrosine hydroxylase activity using (A) mushroom TYR, cell lysates from (B) HEMs, (C) melan‐a cells, and (D) B16F10 cells. The tyrosine activity was determined using 1 mM tyrosine as the substrate in the absence and presence of l‐AHG. All data (n = 3) represent the mean values ± SD. Means with letters (a‐e) in a graph are significantly different from each other at P < .05. HEMs, human epidermal melanocytes; l‐AHG, 3,6‐anhydro‐l‐galactose; SD, standard deviation; TYR, tyrosinase