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. 2018 Jun 19;57(10):1342–1357. doi: 10.1002/mc.22849

Figure 2.

Figure 2

EWS cells use glutamine as a nutrient source. (A) Relative cell viability of TC32, SKNMC, and HCT116 cells measured after 24 or 48 h of serine‐glycine deprivation as indicated (mean ± SEM of three independent experiments). (B) Relative cell viability of TC32, SKNMC, and HCT116 cells measured after 24 or 48 h of glucose deprivation as indicated (mean ± SEM of three independent experiments). (C) Relative cell viability (mean ± SEM of three independent experiments) of TC32, SKNMC, and HCT116 cells measured after 24 or 48 h of glutamine deprivation as indicated. (D) Relative cell viability of TC32 and SKNMC cells measured after the indicated treatment with L‐DON (mean ± SEM of three independent experiments). (A‐D) Two‐way ANOVA (adjusted P‐value) *P < 0.05, **P < 0.01, ***P < 0.001