Figure 4.

Effects of anabasum and prednisolone on cytokine and chemokine biosynthesis. Healthy male volunteers were randomized to receive by oral route either placebo, 5 mg anabasum, 20 mg anabasum, twice daily, or 15 mg prednisolone once daily for 4 days. On the fourth day, acute inflammation was triggered by intradermal injection of 1.5 × 10⁷ UV‐killed E. coli (UVkEc) suspended in 100 μL of saline in both forearms. Inflammatory exudate at the injection site was acquired into a suction blister raised after 4 h (onset phase) on one forearm and after 10 h (resolution phase) on the contralateral forearm. Cytokines and chemokines in the inflammatory exudate were measured by multiplex enzyme‐linked immunosorbent assay (ELISA). The concentrations of cytokines and chemokines in the inflammatory exudate at 4 h and 10 h are shown. Data expressed as individual values with mean ± SD. n = 5/group. *P < 0.05, **< 0.01.