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. 2018 Jul 3;41(10):2342–2356. doi: 10.1111/pce.13357

Figure 1.

Figure 1

Natural variation in 349 wild Arabidopsis accessions for PLANT DEFENSIN1.2 (PDF1.2) transcript levels after treatment with methyl JA (MeJA) or a combination of MeJA and either salicylic acid (SA) or abscisic acid (ABA). (a) PDF1.2 transcript levels relative to those of the constitutively expressed Arabidopsis reference gene PP2AA3 (At1g13320) in leaves of control (blue dots) or MeJA‐treated plants (pink dots). (b) PDF1.2 transcript levels in leaves of control (blue dots) or a combination of MeJA and SA (red dots). (c) PDF1.2 transcript levels in leaves of control (blue dots), or a combination of MeJA and ABA (green dots). (d) Fold change in PDF1.2 expression in MeJA + SA‐ over MeJA‐treated plants. (e) Fold change in PDF1.2 expression in MeJA + ABA‐ over MeJA‐treated plants. For each accession, gene expression analyses were performed 24 hr after hormone treatment of 4‐week‐old plants. In panels a–c, accessions are similarly sorted on the level of PDF1.2 expression in the respective control plants (blue dots). In panels d–e, accessions are sorted on the magnitude of the effect of the respective hormone treatments. Hence, the order of the accessions differs in these panels. (f) The Venn diagram shows the overlap between the accessions that display repression (green) or enhancement (purple) of MeJA‐induced PDF1.2 by SA and repression (blue) or enhancement (pink) of MeJA‐induced PDF1.2 by ABA. Only accessions showing >2‐fold change in PDF1.2 expression in the combination treatment with MeJA over the treatment with MeJA alone are included in the comparison (305 accessions for MeJA + SA and 333 accessions for MeJA + ABA)