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. 2018 May 22;224(2):e13085. doi: 10.1111/apha.13085

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© 2018 The Authors. Acta Physiologica published by John Wiley & Sons Ltd on behalf of Scandinavian Physiological Society

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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K8^+/− mouse islet cells express less K8 and K18 than K8^+/+ islets. A, Western blot for K8, K18 and Hsc70 (loading control) of protein lysate from pancreatic islets isolated from K8^+/+ and K8^+/− mice (lane 1‐3, K8^+/+; lane 4‐6, K8^+/−). B, Quantification of immunoblotting showed a statistically significant fivefold decrease in K8 protein levels in K8^+/−‐isolated pancreatic islets compared to K8^+/+ islets when normalized to Hsc70 levels. *P < .05, error bars represent mean ± SEM. C, Quantification of immunoblotting showed a significant twofold decrease in K18 protein levels in K8^+/−‐isolated pancreatic islets compared to K8^+/+ islets when normalized to Hsc70 levels. †P < .001, error bars represent mean ± SEM. D, Immunostaining of K8 or K18 (green), insulin (red, used as a marker for islets) and nuclei (blue, colours seen in the merged images) of pancreatic sections from K8^+/+ and K8^+/− mice shows a less dense islet keratin network in K8^+/−.mice compared to K8^+/+ mice. Scale bar: 100 μm