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. 2018 May 22;224(2):e13085. doi: 10.1111/apha.13085

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© 2018 The Authors. Acta Physiologica published by John Wiley & Sons Ltd on behalf of Scandinavian Physiological Society

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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MFN2 protein levels and GLUT2 protein levels and localization are unaltered in K8^+/− mice. A, Western blot analysis for MFN2 and Hsc70 (loading control) of protein lysate from pancreatic islets isolated from K8^+/+ and K8^+/− mice (lanes 1‐3, K8^+/+; lanes 4‐6, K8^+/−) showed no changes in the protein levels of MFN2 between the genotypes. B, Quantification of MFN2 immunoblotting from (A) using Hsc70 as loading control showed no significant changes in protein levels of MFN2 between K8^+/+ and K8^+/− mice. C, Immunostaining of pancreatic sections from K8^+/+ and K8^+/− mice for GLUT2 (red) and nuclei (blue) showed equal intensities of GLUT2 staining on the β‐cell membranes. Scale bar: 5 μm. D, Western blot for GLUT2 and Hsc70 (loading control) of isolated islet lysates showed no differences in GLUT2 amounts in total lysates of K8^+/+ and K8^+/− pancreas (lanes 1‐3, K8^+/+; lanes 4‐6, K8^+/−). (E) Quantification of GLUT2 immunoblotting from (D) balanced to loading control Hsc70 showed no significant changes between the genotypes