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. 2018 May 16;179(2):381–393. doi: 10.1111/bjd.16255

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© 2017 The Authors. British Journal of Dermatology published by John Wiley & Sons Ltd on behalf of British Association of Dermatologists.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Epithelial‐only skin constructs grown on cell‐assembled extracellular matrices (ECMs). (a) Viability assay of cultured keratinocytes (KCs) on different fibroblast‐derived matrices (mean ± SD). Immunofluorescence images of (b) collagen IV (COL4) and (c) collage VII (COL7) in entire skin and epithelial‐only constructs cultured on coating matrix, or dermal papilla fibroblast (DPfi), papillary fibroblast (Pfi) and reticular fibroblast (Rfi)‐derived ECM. (d) Ultrastructural analysis of the basement membrane (arrows) in the epithelial‐only skin constructs (n = 3). Scale bars for (b, c) are 50 μm. Scale bars for (d) are 400 nm. RFU, relative fluorescence unit; CTR, control; DAPI, 4’,6‐diamidino‐2‐phenylindole.