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. 2018 May 16;179(2):381–393. doi: 10.1111/bjd.16255

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© 2017 The Authors. British Journal of Dermatology published by John Wiley & Sons Ltd on behalf of British Association of Dermatologists.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Extracellular matrix (ECM) composition. (a) Venn diagram showing distribution of 12 ECM components in matrices assembled from dermal papilla fibroblast (DPfi), papillary fibroblast (Pfi) and reticular fibroblast (Rfi). Representative immunofluorescence images of (b) collagen I (COL1), (c) collagen VI (COL6), (d) tenascin (TNC) and (e) thrombospondin 1 (THBS1) in ECMs generated by DPfi, Pfi or Rfi cells after 10 days in ascorbic acid‐supplemented medium. (f–i) Quantification of immunofluorescence is plotted in graphs as the mean ± SD (n = 3). *P < 0·05. Scale bars in (b–e) are 50 μm. COL1A1, α1 chain collagen I; COL6A2, α2 chain collagen VI; COL1A2, α2 chain collagen II; VTN, vitronectin; FN1, fibronectin 1; COL6A1, α1 chain collagen VI; COL6A3, α3 chain collagen VI; FBLN2, fibulin 2.