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. 2018 Sep 24;84(3):386–400. doi: 10.1002/ana.25267

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© 2018 The Authors. Annals of Neurology published by Wiley Periodicals, Inc. on behalf of American Neurological Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Injection with live‐attenuated Japanese encephalitis vaccine (LA‐JEV) leads to the production of anti–acetylcholine receptor antibodies (AChRAbs). (A) The reactivity of the serum from LA‐JEV/RNA‐dependent RNA polymerase (RdRp)/complete Freund adjuvant (CFA)‐immunized mice with the TWTYDGS epitope was tested by dot blot. LA‐JEV/RdRp‐immunized mouse serum could react with TWTYDGS conjugated to ovalbumin (Pep+OVA). (B) The samples of Pep+OVA were 2‐fold serial diluted and spotted onto a piece of nitrocellulose membrane. RdRp‐immunized mouse serum could react with Pep+OVA at a concentration of 3.13 µg/ml. (C) The serum of childhood onset myasthenia gravis (CMG) patients could react with the TWTYDGS epitope, whereas that of adult myasthenia gravis (MG) patients and healthy controls were less likely to react with the TWTYDGS epitope. OVA = ovalbumin.