Fig 8. High molecular weight Ref(2)P is more abundant in Gba1b mutant EVs.

Whole-fly homogenates and isolated extracellular vesicle extracts from Gba1b mutants and controls were subjected to western blot analysis using an antibody to Ref(2)P. Gba1b = Gba1b^ΔTT/Gba1b^ΔTT; control = Gba1b^rv/ Gba1b^rv. (A) Arrowhead indicates monomeric Ref(2)P; bracket indicates high molecular weight Ref(2)P. (B) Quantification of monomeric Ref(2)P. (C) Quantification of high molecular weight Ref(2)P. At least three independent experiments were performed. Representative image is shown. Error bars represent SEM. *p < 0.01 by Student t test.