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. 2018 Oct 1;24(4):656–668. doi: 10.5056/jnm18037

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© 2018 The Korean Society of Neurogastroenterology and Motility

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

MicroRNA-200a (miR-200a) more strongly inhibited the expression of cannabinoid receptor 1 (CNR1) and serotonin transporter (SERT) in diarrhea-predominant irritable bowel syndrome colonic epithelial cells (CECs) than si-CNR1 and si-SERT. (A) Quantitative real-time polymerase chain reaction (qRT-PCR) showed successful transfection of miR-200a, si-CNR1, and si-SERT (*P < 0.05, ANOVA), and there were no significant differences between si-CNR1 group and the si-SERT group (NS, least significant difference [LSD]). (B) Western blot showed that CNR1 and SERT were lowly expressed in the miR-200a and si-CNR1/si-SERT groups compared to the model group. Furthermore, CNR1 and SERT were significantly downregulated in the miR-200a group compared to the si-CNR1 and si-SERT groups. (C, D) Quantitative RT-PCR shows similar results to western blot (*P < 0.05, ANOVA; NS, LSD).