Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) |
Par3 | PMID: 10934475 | accession number AY026057 |
|
| Gene (Xenopus laevis) |
Par3 | PMID: 25070955 | Xl.16888 | Brian Mitchell lab |
| Gene (X. laevis) |
Prickle3 (Pk3) | PMID: 27062996 | GenBank BC154995 |
Sergei Sokol lab |
| Gene (X. laevis) |
Vangl2 | PMID: 27658614 | GeneID: 398271 | Sergei Sokol lab |
| Gene (Aquifex aeolicus) |
Biotin Ligase (BL) | PMID: 26912792 | Sergei Sokol lab | |
| Genetic reagent (X. laevis) |
Par3 morpholino, Par3MOATG |
this paper | ||
| Genetic reagent (X. laevis) |
Par3 morpholino, Par3MO5'UTR |
this paper | ||
| Genetic reagent (X. laevis) |
Vangl2 MO | PMID: 26079437 | ||
| Cell line (Homo sapiens) |
HEK293T | ATCC | RRID:CVCL_0063 | |
| Antibody | anti-PKCζ (rabbit polyclonal, C20) |
Santa Cruz | RRID:AB_2300359 | (1:200 IHC) |
| Antibody | anti-Biotin (goat polyclonal) |
Cell Signaling | RRID:AB_10696897 | (1:3000 IB) |
| Antibody | anti-β-Catenin (rabbit polyclonal) |
Sigma | RRID:AB_476831 | (1:200 IHC) |
| Antibody | anti-FLAG (mouse monoclonal, M2) |
Sigma | RRID:AB_439685 | (1:1000 IB) |
| Antibody | anti-GFP (mouse monoclonal, B2) |
Santa Cruz | RRID:AB_627695 | (1:100 IHC, 1:4000 IB) |
| Antibody | anti-GFP (rabbit polyclonal) |
Invitrogen | RRID:AB_221569 | (1:600) |
| Antibody | anti-HA (mouse monoclonal, 12CA5) |
NA | RRID:AB_2532070 | (1:100 IHC, 1:1000 IB) |
| Antibody | anti-HA (rabbit polyclonal) |
Bethyl Laboratories |
RRID:AB_67465 | (1:3000 IB) |
| Antibody | anti-Myc (mouse monoclonal, 9E10) |
RRID:CVCL_G671 | (1:60 IHC) | |
| Antibody | anti-Par3 (rabbit polyclonal) |
Millipore | RRID:AB_11213581 | (1:200 IHC, 1:4000 IB) |
| Antibody | anti-Vangl2 (rabbit polyclonal) |
PMID: 25910938 | RRID:AB_2744499 | (1:100 IHC, 1:500 IB) |
| Antibody | anti-ZO1 (mouse monoclonal) |
Invitrogen | RRID:AB_2533147 | (1:200 IHC) |
| antibody | anti-ZO1 (rabbit polyclonal) |
Zymed | RRID:AB_138452 | (1:200 IHC) |
| Recombinant DNA reagent |
BL-Pk3 | this paper | (plasmid) | |
| Recombinant DNA reagent |
BL-Vangl2 | this paper | (plasmid) | |
| Recombinant DNA reagent |
GFP-C1 | PMID: 22778024 | (plasmid) | |
| Recombinant DNA reagent |
GFP-CAAX | PMID: 24818582 | (plasmid) | |
| Recombinant DNA reagent |
GFP-Dvl2 | PMID: 15720724 | (plasmid) | |
| Recombinant DNA reagent |
GFP-Par3 | PMID: 25070955 | (plasmid) | |
| Recombinant DNA reagent |
GFP-Par3N | PMID: 25070955 | (plasmid) | |
| Recombinant DNA reagent |
RFP-Par3N | PMID: 25070955 | (plasmid) | |
| Recombinant DNA reagent |
mCherry | PMID: 19096028 | (plasmid) | |
| Recombinant DNA reagent |
Myc-Par1T560A | PMID: 17993468 | (plasmid) | |
| Recombinant DNA reagent |
Myc-Par3 | this paper | (plasmid) | |
| Recombinant DNA reagent |
Myc-Par3[1-271] | this paper | (plasmid) | |
| Recombinant DNA reagent |
Myc-Par3[272-544] | this paper | (plasmid) | |
| Recombinant DNA reagent |
HA-Par3[272-544] | this paper | (plasmid) | |
| Recombinant DNA reagent |
HA-Par3[545-756] | this paper | (plasmid) | |
| Recombinant DNA reagent |
Myc-Par3[545-756] | this paper | (plasmid) | |
| Recombinant DNA reagent |
Myc-Par3[757–1035] | this paper | (plasmid) | |
| Recombinant DNA reagent |
Myc-Par3[934–1334] | this paper | (plasmid) | |
| Recombinant DNA reagent |
Myc-Par3∆∆ | this paper | (plasmid) | |
| Recombinant DNA reagent |
HA-RFP-Pk3 | this paper | (plasmid) | |
| Recombinant DNA reagent |
HA-Vangl2 | PMID: 27658614 | (plasmid) | |
| Recombinant DNA reagent |
GFP-Pk3 | PMID: 27062996 | (plasmid) | |
| Recombinant DNA reagent |
FLAG-Pk3 | PMID: 27062996 | (plasmid) | |
| Recombinant DNA reagent |
FLAG-GFP-Pk3 | PMID: 27062996 | (plasmid) | |
| Recombinant DNA reagent |
FLAG-Pk3∆PET | PMID: 27062996 | (plasmid) | |
| Recombinant DNA reagent |
FLAG-GFP-Pk3∆PET | PMID: 27062996 | (plasmid) | |
| Recombinant DNA reagent |
turboFP635 | this paper | (plasmid) | |
| Immunoprecipitation reagent | Myc-Trap beads | Chromotek |