Skip to main content
. 2018 Oct 3;7:e39514. doi: 10.7554/eLife.39514

Figure 4. Deletion of the protein translocases disrupts the T3SS-dependent intimate attachment to the host PM, and the formation of the translocon.

(A) Percentage of minicells attached to the host membrane via needle-membrane contact. Data were compared using a chi-squared test. (B, C) Central slices from tomograms showing the ΔsipBCD injectisomes interacting with the host PM. (E, F) Central slices from tomograms showing the ΔsipB injectisomes interacting with the host PM. (H, I) Central slices from tomograms showing the ΔsipD injectisomes interacting with the host PM. Blue arrows indicate needles attached to the host PM. Red arrows indicate unattached needles. (D, G, J) Schematic models depicting needle-attachment patterns from three mutants, respectively.

Figure 4.

Figure 4—figure supplement 1. Gallery of snapshots from host-free and host-interacting S. Typhimurium minicells.

Figure 4—figure supplement 1.

Central slices from representative tomograms showing (A) host-free and (B) host-interacting S. Typhimurium minicells from WT, ΔsipB, ΔsipD, ΔsipBCD, and ΔspaO strain.