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. Author manuscript; available in PMC: 2019 Jun 15.
Published in final edited form as: J Org Chem. 2018 May 29;83(12):6307–6314. doi: 10.1021/acs.joc.8b00169

Fig. 2.

Fig. 2

(A) SDS-PAGE for conjugation of O-PEG–chlorin to lysozyme in DMSO. (lane 1) Molecular weight marker. (2) Free lysozyme. (3) Crude product reaction (1 : 1). (4) Free SPEG–chlorin. (5) Crude product reaction (1 : 1). (B) SDS-PAGE for conjugation of S-PEG–chlorin to lysozyme in DMSO. (lane 1) Molecular weight marker. (2) Free lysozyme. (3) Crude product reaction (1 : 1). (4): Free SPEG–chlorin. (5) Crude product reaction (1 : 1). Lanes 1–3 visualization of protein after staining with Coomassie blue. Lanes 4 and 5 visualization of chlorin fluorescence after excitation at 365 nm.