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Timeline. Completion of the full protocol occurs in a series of stages: (I) Handling and housing room acclimation (7 days), (II) Habituation (2 days), (III) Training (3 days), (IV) Home cage rest (2 days), (V) Baseline stimulus-response testing (7 days), (VI) Induction of dependence and withdrawal (time frame dependent on protocol used), and (VII) Dependence and withdrawal stimulus-response testing (7 days).
