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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Nanomedicine. 2018 Apr 8;14(4):1301–1313. doi: 10.1016/j.nano.2018.03.010

Figure 3.

Figure 3

Cellular uptake studies and effects of NPs on intracellular localization of PTX and NuBCP-9. (A) Upper panel. MCF-7 cells were treated with NPs encapsulated with the RhoB or Coumarin-6 dyes for 12 h. After washing, the cells were fixed and visualized by confocal microscopy (60X magnification). Lower panel. MCF-7 cells were treated with NPs encapsulated with the RhoB or Coumarin-6 dyes for 12 h and cellular uptake was quantified by FACS. (B) Upper panel. MCF-7 cells were treated with FITC-labeled NuBCP-9/NPs for 12 h. After washing, the cells were fixed and visualized by confocal microscopy (100X magnification). Mitochondria were stained with mitochondria selective Mitotracker dye. Green signal, FITC-NuBCP-9. Red signal, Mitotracker. Yellow/ orange signal, colocalization of NuBCP-9 and Mitotracker. Scatter plot graph of Pearson’s correlation coefficient (r) Lower panels. MCF-7 cells were treated with NPs encapsulated with both FITC-labeled PTX and Rho-NuBCP-9 for 12 h. After washing, the cells were fixed and visualized by confocal microscopy (100X magnification). Green signal, FITC-labeled PTX. Red signal, RhoB-labeled NuBCP-9. Yellow/orange signal, colocalization of PTX and NuBCP-9. Scatter plot graph of Pearson’s correlation coefficient (r).