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Pyruvate concentration. Representative graphic of the pyruvate concentration in different culture conditions. Each point represents the mean ± SD (n = 3). Statistical analysis by two-way ANOVA–Bonferroni's post-test. *p < 0.05 (14 °C + 1%M vs. all other conditions); **p < 0.01 (18 °C + 1.5%M vs. 22 °C + 1%M, 14 °C + 2%M, 14 °C + 1%M);***p < 0.01 (22 °C + 2%M vs. 22 °C + 1%M, 14 °C + 2%M, 14 °C + 1%M).
