Table 1.
Primer and PCR information
| Lipopeptide | Target gene | Primer name | Sequence (5ʹ–3ʹ) | Annealing temp. (°C) | Product size (bp) |
|---|---|---|---|---|---|
| Surfactin | sfp gene | Sfp-F Sfp-R |
ATGAAGATTTACGGAATTTA TTATAAAAGCTCTTCGTACG |
46 | 675 |
| Surfactin | SrfD | SrfAD-F SrfAD-R |
CCGTTCGCAGGAGGCTATTCC CGCCCATCCTGCTGAAAAAGCG |
60 | 1300 |
| Fengycin | FenB | FenB-R FenB-R |
CTATAGTTTGTTGACGGCTC CAGCACTGGTTCTTGTCGCA |
53 | 1600 |
| Iturin | ItuA | ItuA-F ItuA-R |
ATGTATACCAGTCAATTCC GATCCGAAGCTGACAATAG |
46 | 1047 |
| Iturin | ItuC | ItuC-F ItuC-R |
CCCCCTCGGTCAA GTGAATA TTGGTTAAGCCCTGATGCTC |
54 | 594 |
| Bacillomycin | BamA | BamA-F | AAAGCGGCTCAAGAAGCGAAACCC | 63 | 1200 |
| BamA-R | CGATTCAGCTCATCGACCAGGTAGGC |
PCR primers were determined from the genome sequence of B. siamensis JFL15 (GenBank genome accession number LFWQ00000000)