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Basal cytokine release of ALL cells does not correlate with OX40 expression intensity. PBMC of ALL patients were cultured for 24 (IL-6) or 6 hours (TNF and IL-8) before cytokine levels in culture supernatant were determined by ELISA. Release of IL-6 (left), TNF (middle), or IL-8 (right) and corresponding OX40 surface expression levels (SFI) are shown for individual patient samples. Correlation coefficient r was calculated using GraphPad Prism 7 software.
