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. 2018 Oct 8;8:14914. doi: 10.1038/s41598-018-33267-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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P. gingivalis-induced experimental periodontitis. (A) Histological hematoxylin-eosin staining of periodontium of non-treated site on the first molar of mice (Hematoxylin & Eosin) (10x magnification). (B) Morphological characteristics of periodontium of P. gingivalis ligature treated site on the first molar of mice (Hematoxylin & Eosin) (10x magnification). (C–H) Immunofluorescence staining for localization of Cytokeratin 14, Pan Cytokeratin and E-cadherin expression in epithelial tissue (red) and Collagen IV expression (green) in the periodontium (10x magnification). (I–P) Immunofluorescence staining for localization of Integrin β1, vimentin, laminin α5 and anti-P. gingivalis expression in the periodontium (green) at diseased site (10x magnification: I–M and O; 20x magnification: N,P). In all immunofluorescence images, nucleus has been stained by DAPI in blue.