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. 2018 Oct 8;8:14961. doi: 10.1038/s41598-018-33264-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bacterial growth in LB batch cultures (in vitro). (a) Bacterial growth measured as bacterial counts (log₁₀ CFU/ml), ori:ter_qPCR and ori:ter_mic. ATCC 25922 and ALO 4783 were tested in parallel and showed similar growth; hence, results from both strains are pooled in the data sets. Bland-Altman agreement between ori:ter_qPCR and ori:ter_mic was good (Supplementary Fig. S1). 6 h, n = 12; all other time points, n = 6. (b) Comparisons of ATCC 25922 and ALO 4783 grown in LB batch cultures (in vitro) revealed no growth retardation due to transgene insertions. Growth measured as OD₆₀₀. Both strains exerted doubling times of 25 minutes during exponential growth. (c) Bacterial cell length (µm) (used as proxy for cell mass) correlate with oriC/cell. Data represent pooled microscopically detected cells (ALO 4783). n = 500 at all time points, with the exception of 2 h, where n = 147. Data are presented as mean ± SD. Dotted line represents the limit of detection for bacterial count measurements. Time (h) represents hours of incubation; inoculum is presented as 0 h. r: Pearson’s correlation coefficient.