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. 2018 Oct 8;8:14951. doi: 10.1038/s41598-018-33030-9

Figure 2.

Figure 2

Differentiation potentials of PSA-NCAM positive neuronal progenitors in rat brains after MCAO. To determine the differentiation potentials of PSA-NCAM positive cells, double immunofluorescence staining of PSA-NCAM and cell type specific markers (GFAP for astrocytes, and β-tubulin for neuronal markers) were done, as described in the Materials and Methods. Representative photomicrographs of β-tubulin (a and d) and PSA-NCAM (b and e) double immunofluorescence staining of day 14 MCAO rat brains, and their merged pictures (c and f) are shown in (A), where (a–c) are photomicrographs of core, and (d–f) are of penumbra area. Representative photomicrographs of GFAP (a and d) and PSA-NCAM (b and e) double immunofluorescence staining of day 14 MCAO rat brains, and their merged pictures (c and f) are shown in (B), where (a–c) are photomicrographs of core, and (d–f) are of penumbra area. GFAP+ and β-tubulin+ cells at day 14 were counted at X400 magnification and shown in (C,E), respectively. Double positive cells were counted and their percentage over total GFAP and β-tubulin were calculated, and shown in (D,F), respectively. Numerical data are presented here as mean ± standard error of means (n = 5). Statistical significance was denoted as follows: *p < 0.01 vs PBS group. Scale bar = 20 μm.