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. 2018 Oct 8;8:14951. doi: 10.1038/s41598-018-33030-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differentiation potentials of transplanted B10 cells in MCAO rat brain. To determine the differentiation potentials of B10 cells in MCAO rat brain, double immunofluorescence staining of human nuclei (HuN) and cell type specific markers (GFAP for astrocytes, and β-tubulin for neuronal markers) were done in day 3 MCAO rats. Representative photomicrographs of HuN and β-tubulin double immunofluorescence staining of PBS treated and B10 transplanted rats are shown in (A), and double immunofluorescence staining of HuN and GFAP of B10 transplanted rats are shown in (B). Hoechst staining was done to identify the nuclei.