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. 2018 Oct 8;8:14951. doi: 10.1038/s41598-018-33030-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of B10 transplantation on mRNA expression of endogenous migration regulators in MCAO rat brain. To determine the effects of B10 transplantation on the regulation of neuronal progenitor’s migration, the mRNA expression of migration regulators was analyzed. Total RNA was isolated from core, penumbra and contralateral cortex of PBS-treated and B10-transplanted rat at day 3 (A), day 7 (B) and day 14 (C) after MCAO, and mRNA expression of migration regulators was analyzed by a real-time PCR system, as described in Materials and Methods. The PCR data is presented as mRNA level relative to a calibrator sample, and expressed as mean ± SD of 5 samples in a group. GAPDH mRNA was used as loading control. Statistical significance was denoted as follows: *p < 0.01 vs PBS group of same area and same time point.