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. 2018 Oct 8;8:14951. doi: 10.1038/s41598-018-33030-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification and identification of SDF-1 expressing cell types in rat brains after MCAO. (A) After immunofluorescence staining of SDF-1, positive cells were counted in 5 random field of designated areas at X400 magnification. Three tissue sections of 2 mm apart were used to count the cells, and the average was considered as positive cell number of that rat. The data presented here as average ± SD (n = 5), and the statistical significance was evaluated by one-way ANOVA followed by Student-Newman-Keuls Post hoc analysis. *p < 0.05. (B) To determine the localization, double immunofluorescence staining of SDF-1 and macrophage/microglia marker ED1 was done, as described in the Materials and Methods. Representative photomicrographs of SDF-1 (a,d,g and j) and ED-1 (b,e,h and k), and their merged picture (c,f,i and l) in the core (a–f) and penumbra (g–l) areas of PBS-control (a–c and g–i) and B10-transplanted (d–f and j–l) rats are shown. (B) To determine SDF-1 expression in transplanted B10 cells, double immunofluorescence staining of SDF-1 and human nuclei (to identify human origin B10 cells) was done in B10 transplanted rats 3 days after MCAO. Representative photomicrographs of SDF-1 (a and d), human nuclei (b and e) and their merged pictures (c and f) in the core (a–c) and penumbra (d–f) are shown. Scale bar = 50 μM.