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. 2018 Oct 8;8:14916. doi: 10.1038/s41598-018-33092-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Body physiology and pathological indices: (a) relative body weight changes and (b) disease activity index (DAI) scores monitored every two days over the 10 days (MDSS and QDSS mice was treated with 5 days DSS exposure in drinking water and followed by another 5 days tap water); (c) colon lengths, (d) spleen weights, (e) histological analysis and (f) histological severity scores of the different treatment mice. Comparisons of various anatomical measurements among four groups were made by two-way analysis of variance (ANOVA), followed by Fisher’s LSD post hoc tests. ^*Indicates significant difference with P < 0.05, ^**Indicates P < 0.01 and ^***Indicates P < 0.001; NS indicates lack of statistically significant difference. In (a) and (b) the statistically differences were only marked within the DSS-treated or Non-DSS control groups (MC vs. QC; MDSS vs. QDSS).