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. 2018 Oct 8;8:14904. doi: 10.1038/s41598-018-33298-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ELISA tests of anti-CPEN, CNEN, and Atr-I sera. (A–C) show the boxplots results for ELISA plates coated individually by CPEN, CNEN, and Atr-I, respectively. |Plates were incubated for 1 h with the respective sera at 37 °C followed by another round of 3× washing before incubation with a respective secondary antibody for 1 h. An OPD substrate was added for ~20 minute incubation, and the reaction was stopped with H₂SO₄ prior plate reading. The black lines within the boxes correspond to medians. All the samples marked with (*) show to be significantly different for a p < 0.05, when comparing by the t test available in software R. (D) shows antibody binding over 9 doses (x-axis represents days).