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. 2018 Oct 8;8:14965. doi: 10.1038/s41598-018-33282-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glutamatergic and GABAergic glioma cells. (A,B) Westernblot of GSC lines and control samples (breast cancer cell line MCF7, classical glioma cell lines T98G, U87). Blots were probed with antibodies against GAD67, synaptophysin, SV2A and vGlut1. Actin was used as loading control. (C) Immunocytochemistry against vGlut1 and synaptophysin. DAPI was used to label the nuclei. (D,E) Correlation of synaptophysin expression with vGlut1 (Pearson r = 0.92, p < 0.001) and GAD67 (Pearson r = 0.36, p = 0.001) using mRNA expression data from the RemBranDT database (accessed: 09 January 2018).