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. 2018 Oct 8;9:4118. doi: 10.1038/s41467-018-06409-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — eCB-tLTP does not depend on D₂R expressed by dopaminergic neurons. a–d Experiments of plasticity expression in 6-ODHA-ablated dopaminergic cells in the MFB. a Scheme of the protocol showing the time course of the in vivo 6-OHDA lesions and ex vivo electrophysiological recordings. b Unilateral 6-OHDA injection in MFB led to degeneration of dopaminergic nigral neurons, illustrated by a loss of their striatal afferences as illustrating by the summary bar graph of TH immunostaining quantification. Sham-operated rats display equivalent TH staining in both striata. c 10 post-pre pairings induced tLTP in sham-operated (n = 7, 7/7 cells showed tLTP) rats whereas tLTP was impaired in 6-OHDA-lesioned rats (n = 7, 1/7 cells showed tLTP), and d rescued with a D₂R agonist, quinpirole (10 µM, n = 6, 5/6 cells showed tLTP). e, f STDP expression in selective cKO for D2R expressed by dopaminergic neurons. e In mice in which D₂R was specifically knocked-out in dopaminergic neurons (DAT-Cre^+/−::Drd2^LoxP/LoxP mice) tLTP was induced with 15 post-pre pairings (n = 6, 6/6 cells showed tLTP). f tLTP was observed in mice serving as the Cre driver control (DAT-Cre^+/−::Drd2^Wt/Wt mice, n = 8; 6/8 cells showed tLTP). Representative traces are the average of 15 EPSCs during baseline (black traces) and 45 min after STDP protocol (grey traces). Vertical grey dashed line indicates the STDP protocol. Error bars represent sem. *p < 0.05; **p < 0.01; ***p < 0.001; ns: not significant by Wilcoxon Signed rank test (b) or one sample t-test (c–f)