Fig. 8. Changes in expression of the mesenchymal marker vimentin in cells expressing Vav1-mutant proteins.
Vimentin expression (red) was detected by immunofluorescence in NIH3T3 cells stably expressing pcDNA3, Vav1, oncVav1, E59K, D517E, and L801P. Nuclei are stained with DAPI (blue). Representative photographs indicating the differences in morphology of the cells were taken with Nikon eclipse 90i confocal microscope and analyzed by the NES element program